Differential Pulse Voltammetric Determination of Clenbuterol with Fullerene Modified Electrode

Fullerene (C60, 1.08 mg) was dispersed in CH2Cl2 (10 mL), and dropped on the surface of glassy carbon electrode (GCE), then activated in 1 mol·L-1 KOH solution to prepare C60 modified GCE (C60/GCE). Electrochemical behavior of the modified electrode was studied by cyclic voltammetry. As shown by the experimental results, a pair of quasi-reversible oxidation and reduction peaks was observed in 5 mmol·L-1 K3Fe(CN)3 solution at the modified electrode, and the peak currents were found to be higher than those obtained at bare GCE and unactivated modified electrode. Based on these findings, a selective method for determination of clenbuterol (CLB) by differential pulse voltammetry was proposed. Linear relationship between values of oxidation peak current and concentration of CLB was kept in the range of 0.1-20 μmol·L-1 with detection limit (3S/N) of 3.26×10-2μmol·L-1. The proposed method was used in the analysis of samples of urine, and values of recovery found were in the range of 99.5%-110%.