LCMS/MS Determination of 9 Banned Aromatic Amines in Cosmetics

LCMS/MS was applied to the determination of 9 banned aromatic amines, i.e. benzidine, 4,4′oxydianiline, oanisidine, 3,3′dimethylbenzidine, 2,4,5trimethylaniline, 4,4′thiodianiline, 5nitrootoluidine, 3,3′dichlorobenzidine and 4aminoazobenzene in cosmetics. The sample was extracted with 1% (φ) trichloroacetic acid solution and acetonitrile. An aliquot of the supernatant was purified on solid phase extraction column, the eluate was separated by using C18 column (50 mm×4.6 mm, 1.8 μm) as stationary phase, and mixtures of 0.05% (φ) formic acid solution and acetonitrile (containg φ 0.05% formic acid) with different ratios was used as mobile phase in gradient elution. ESI+ and MRM were adopted in MS/MS determination. Linearity range of 3,3′dichlorobenzidine was found between 0.5-50 mg·kg-1 with detection limits (3S/N) of 0.15 mg·kg-1. Linearity ranges of the other 8 aromatic amines were found same between 0.2-50 mg·kg-1 with same detection limit (3S/N) of 0.05 mg·kg-1.Values of recovery found by standard addition method were in the range of 78.3%-103%, and values of RSD′s (n=6) less than 6.0%.