Determination of Glucose, Fructoes and Sucrose in Seabuckthorn Fruit Honey by HPLC with ELSD

HPLC was applied to the determinations of glucose, fructoes and sucrose in seabuckthorn fruit honey. The 3 saccharides were separated on Zorbax-NH2 (4.6 mm×250 mm, 5 μm) chromatographic column with a mixture of acetonitrile and water (90+10) as mobile phase, and their contents determined by evaporation light scattering detector (ELSD). Linear relationship between logarithmic values of peak area and mass concentration of the 3 saccharides were obtained in the same range of 10-1 000 mg·L-1 and with same detection limit (3S/N) of 10 mg·L-1. The values of average recovery found by standard addition method were in the range of 95.0%-105.0%, with values of RSD′s (n=5) less than 2.5%.