HPLC Determination of Aristolochic Acid A in Cosmetic

A method of HPLC for the determination of aristolochic acid A in cosmetics was proposed. The cosmetic sample was extracted twice ultrasonically with methanol for 20 min each, and the extract was filtered on 0.45 μm filtering membrane. In the separation Eclipse XDB-C18 column was used as stationary phase and a mixture of methanol and 0.5% (φ) acetic acid solution (70+30) was used as mobile phase. The eluate obtained was determined with DAD at the wavelength of 250 nm. Linear relationship between values of peak area and mass concentration of aristolochic acid A was kept in the range of 0.6-300 mg·L-1, with detection limit (3S/N) of 0.2 mg·L-1. On the base of cosmetic sample, test for recovery was made by standard addition method; values of recovery found were in the range of 90.6%-102% with RSD′s (n=5) in the range of 0.85%-3.4%.