HPLC Determination of Ascorbyl Palmitate in Foods

High performance liquid chromatography was applied to the determination of ascorbyl palmitate in fatty food,baked food,and ready-to-eat food. The sample was extracted with methanol using citric acid and iso-ascorbic acid as stabilizing agent. The extract obtained was separated on the reversed phase C18 column with mixtures of phosphoric acid (1+99) and mixed solvent of acetonitrile and methanol (1+1) in different ratios as mobile phase in gradient elution. UV-detection at 243 nm with photodiode array detector (PAD) was used in the determination. Linear relationship between values of peak area and mass concentration of ascorbyl palmitate was kept in the range of 0.50-100.0 mg·L-1. Lower limit of determination (10S/N) of the method was found to be 0.005 g·kg-1. Test for recovery were made by standard addition method,giving values of recovery in the range of 85%-104% and RSD′s (n=6) in the range 1.1%-3.9%.