Determination of the Compositional Monosaccharides of Polysaccharides in Aloe by HPGPC,HPLC and ESI-MS

High performance gel permeation chromatography (HPGPC) was applied to the determination of purity and relative molecular mass of polysaccharides in concentrate liquor and powder of aloe.The aloe sample (in liquor or powder state) or its hydrolysis product was pre-column derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP),and the compositional monosaccharides were separated and determined by hyphenation of HPLC with electron spray ionization-mass spectroscopy (ESI-MS).It was found that mannose and glucose were the main compositional monosaccharides of polysaccharides in aloe.