HPLC Determination of 9 Flavones in Rose

A method of HPLC for the determination of 9 flavones in rose was proposed. The sample was extracted with mixture of methanol-H2O (8+2) by speedy and homogeneous pulping. An aliquot of 5.0 mL of the extract was purified by passing through MCI-GEL RP-resin SPE column. Waters Exterra chromatographic column was used as stationary phase with mixtures of methanol and acetic acid (0.5+99.5) solution with different mixing ratios were used as mobile phase in the gradient elution. UV-detection at 358 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 9 flavones were kept in definite ranges. Detection limits (3S/N) found were ranged from 35 to 45 μg·L-1. Values of recovery found by standard addition method were in the range of 96.3%-106%, and values of RSD′s (n=7) in the range of 1.8%-2.8%.