GC-MS Determination of Galanthamine and Lycoramine in Bulb of Lycoris Radiata with Microwave Assisted Extraction

Galanthamine (GLTM) and lycoramine (LCRM) were extracted from the sample of bulbs of Lycoris radiata with ethyl alcohol(95+5) under 70 ℃. The extract obtained was filtered and evaporated to dryness with a rotary evaporator. The residue was dissolved with HCl(2+98) and extracted thrice with CHCl3 to remove impurities. The aqueous phase was adjusted to pH 10 with aq. ammonia and extracted thrice with CHCl3 again. The organic phase was collected and evaporated to dryness. The residue was dissolved with methanol and DB-5MS capillary column was used for separation. One μL was taken as volume of sample introduction. Electron ionization and full-scanning in the range from (m/z) 50 to 550 were adopted in MS analysis. Qualitative analysis was performed by referring to the spectra database of NBS 75K.L and NIST 147. Quantitative analysis was carried out with ［M+］ 286 and ［M+］ 288 for GLTM and LCRM respectively. Tests for recovery and precision were made, giving results of recovery ranged from 93.0% to 106.0% and RSD′s (n=9) of 5.5% (for GLTM) and 2.4% (for LCRM).