HPLC Determination of 8 Synthetic Colorants in Canned Yellow Peach

HPLC was applied to the determination of 8 synthetic colorants,including tartrazine,amaranth,indigotin,ponceau 4R,sunset yellow,allura red,brilliant blue and erythrosine,in canned yellow peach.The sample was extracted with aqueous ethanol-ammonia solution and the extract was purified on Sep-Pak Plus QMA SPE column.The eluate from the column was separated by gradient elution with methanol and ammonium acetate mixed solutions of different ratio on Inertsil ODS-3V (4.6 mm×250 mm,5 μm) column,and determined with diode array detector.Linear relationships between values of peak areas and mass concentrations of the 8 synthetic colorants were obtained in the range of 0.1-20.0 mg·L-1,with their lower limits of determination (10S/N) in the range of 0.25 to 0.5 mg·kg-1.The values of average recovery of 8 synthetic colorants found were in the range of 94.3%-96.2% for tartrazine,93.3%-99.6% for amaranth,77.9%-84.7% for indigotine,95.0%-101.0% for ponceau 4R,94.6%-103.0% for sunset yellow,94.1%-104.0% for allura red,96.4%-104.0% for brilliant blue,85.9%-89.7% for erythrosine.The values of RSD′s (n=6) found were less than 9.0%.