HPLC Determination of Benzalkonium Chloride in Disinfectant Wet Wipes

A method for determination of benzalkonium chloride in disinfectant wet wipes by HPLC was proposed. The sample was extracted ultrasonically with the mobile phase, and then separated on Eclipse XDB-C18 (150 mm×4.6 mm, 5 μm) column, using a mixture of acetonitrile and 70 mmol·L-1 ammonium acetate solution (containing 1% by vol. of triethylamine, adjusted with acetic acid to pH 5.0) in the ratio of 70 to 30 as mobile phase. Photodiode array detections were made at 262 nm. Linear relationships between values of peak areas and mass concentration of benzalkonium chloride was obtained in the range of 100-500 mg·L-1,with limit of detection (3S/N) of 10.2 mg·L-1. The proposed method was used in the analysis of sample of disinfectant wet wipes, values of recovery found by standard addition method were in range of 95.3%-97.8% with RSD′s (n=5) in the range of 2.5%-4.0%.