Page 74 - 理化检验-化学分册2024年第十二期

陈美芳，等：银纳米溶胶表面增强拉曼光谱法快速测定鱼腥草注射液中依诺沙星的含量

Rapid Determination of Enoxacin in Herba Houttuyniae Injection by
Surface-Enhanced Raman Spectroscopy with Silver Nanosol

CHEN Meifang , WANG Xin , CHEN Hongju , AI Lianfeng , MA Li 1*
3*
1
2
1
(1. Hebei Key Laboratory of Environment and Human Health, School of Public Health, Hebei Medical University, Shijiazhuang
050017, China; 2. Xiamen Pers Nano Technology Limited Company, Xiamen 361000, China; 3.Shijiazhuang Customs Technology
Center, Shijiazhuang 050057, China)
Abstract: Using the self-made silver nanosol as the substrate, the method for rapid determination of enoxacin in Herba
Houttuyniae injection by surface-enhanced Raman spectroscopy with silver nanosol was proposed. 1 mL of 1% (mass fraction) silver
nitrate solution was taken and placed in a beaker, and 200 mL of water was added. The mixture was mixed at a rotational speed of
8 000 r · min −1 and heated to boiling. 9 mL of 1% (mass fraction) sodium citrate solution was added dropwise until the solution
turned yellow, and the heating was stopped until the solution turned milky white. The solution was cooled to room temperature,
and centrifuged at a rotational speed of 3 000 r · min −1 for 1 min. The upper solution (40 mL) was collected into the centrifuge
− 1
tube, and 5 mL of water was added. The solution was centrifuged at a rotational speed of 5 000 r · min for 5 min, and the
supernatant was discarded. 5 mL of water was added, and the mixture was ultrasonic treated for 5 min. The resulting silver
nanosol was stored at 4 ℃. The 0. 2 mL of sample was taken, and 0. 1 mL of silver nanosol was added. The mixture was detected
within 200-3 200 cm − 1 using Raman spectrometer. As shown by the results, linear relationship between the corresponding
−1 −1
response intensity at Raman shift of 1 469 cm and mass concentration of enoxacin was found in the range of 0. 5-100 mg · L ,
with detection limit (3S/N) of 0. 043 mg · L −1 . Test for recovery was made on blank sample by standard addition method at 3
concentration levels, giving results in the range of 86. 1%-103%, with RSDs (n=5) of the determined values in the range of
3. 0%-5. 7%.
Keywords: surface-enhanced Raman spectroscopy (SERS); silver nanosol; Herba Houttuyniae injection; enoxacin

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