Page 85 - 理化检验-化学分册2025年第三期

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Determination of 8 Bisphenol Compounds in Source Water in Guangdong
Province by High Performance Liquid Chromatography-Tandem Mass

Spectrometry with Polyamide Solid Phase Extraction

GUAN Wenbi , JIANG Baoying 1
1,2
(1. School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China; 2. Laboratory of Quality &
Safety Risk Assessment for Agro-Product (Zhaoqing), Ministry of Agriculture and Rural Affairs, Zhaoqing 526061, China)

Abstract: A method for determination of 8 bisphenol compounds, including bisphenol A, bisphenol AP, bisphenol
B, bisphenol AF, bisphenol F, bisphenol E, bisphenol Z, and bisphenol C, in source water from Guangdong Province was
proposed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with polyamide solid phase
extraction. The water sample (500 mL) was passed through a solid phase extraction column packed with 1. 00 g of polyamide,
followed by elution with 6 mL of methanol. The eluent (1 mL) was filtered through a 0. 22 µm-nylon needle filter, and the
8 bisphenol compounds were determined by HPLC-MS/MS using Acquity UPLC BEH C 18 column as the stationary phase and
a mixed solution composed of 0. 1 mmol · L −1 ammonium bicarbonate aqueous solution and methanol at different volume ratios
as the mobile phase for gradient elution, with quantitative analysis by matrix matching method. As shown by the results, linear
relationships between the corresponding peak areas and mass concentrations of the bisphenol compounds were kept in definite
−1
ranges, with detection limits (3S/N) in the range of 0. 12-10. 50 ng · L . Test for recovery was made by the standard addition
method, giving results in the range of 73. 3%-111%, and RSDs (n=5) of the determined values ranged from 1. 4% to 12%.
This method was applied to the analysis of 14 tap water samples, 11 river water samples, 3 spring water samples, 2 reservoir
water samples, 2 lake water samples, and 1 well water sample collected from 11 cities in Guangdong Province. It was shown that
the detection rates of the 8 bisphenol compounds ranged from 3. 03% to 87. 88%, with average mass concentrations in the range of
0. 18-290. 22 ng · L −1 and the total average mass concentration of 327. 48 ng · L −1 .
Keywords: high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS); water sample; bisphenol
compound; polyamide

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