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Simultaneous Determination of 17 Hydroxybenzoate Compounds in

Medical Dressing Category Medical Device Products by
High Performance Liquid Chromatography

ZHOU Yuhan , YUAN Rongsui , DUAN Yulin , BIAN Hedong , ZHANG Yuzhen , WEI Jiarong ,
1
2
1
1
3
2
LU Lanjuan , JIANG Shoujun 2*
3
(1. School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China;
2. Guangxi Zhuang Autonomous Region Food and Drug Evaluation and Inspection Center, Nanning 530029, China;
3. Guangxi Minsheng Sino Assessment Group Co., Ltd., Nanning 530007, China)
Abstract: After adding the mixed solution of methanol and acetonitrile at volume ratio of 8∶2 into samples of medical
dressing category medical device products, vortex mixing, ultrasonic extraction, and centrifugal separation were performed.
The supernatant was passed through a 0. 22 μm filter membrane, and the filtrate was introduced into the high performance liquid
chromatograph. Hydroxybenzoate compounds (PBs) were separated by gradient elution with mixed solutions of acetonitrile and
0. 12% (volume fraction) phosphoric acid solution at different volume ratios on the ShimNex CS C 18 column, and quantified
by the external standard method. It was shown that linear relationships between values of mass concentration and peak area of
−1 −1
17 PBs were kept in the range of 1-50 mg · L , with detection limits (3S/N) in the range of 5-18 mg · kg . Test for recovery
was made according to the standard addition method, giving recoveries in the range of 97. 6%-108%, and RSDs (n=6) of the
determined values ranged from 0. 30% to 4. 4%. This method was used to screen 10 batches of actual samples with different
matrices, and the detection rate of PBs was 40%. Methyl p-hydroxybenzoate was detected in 1 batch of samples, and ethyl p-

hydroxybenzoate and propyl p-hydroxybenzoate were detected in 3 batches of samples.
Keywords: medical dressing category medical device product; hydroxybenzoate compound; high performance liquid
chromatography; determination

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