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王 璐，等：QuEChERS结合超高压液相色谱-三重四极杆串联质谱法测定化妆品中5种禁用组分的含量

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Determination of 5 Prohibited Components in Cosmetics by Ultra-High Pressure

Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry
Combined with QuEChERS

WANG Lu, CAI Cuiling, DONG Jie, HOU Yingye, ZENG Guangfeng, XIE Jianjun *
(1. Guangzhou Customs Technical Center, Guangzhou 510623，China; 2. National Import and Export Food Quality Safety Risk

Verification and Evaluation Laboratory (Cosmetics), Guangzhou 510623, China)
Abstract: The method mentioned by the title was proposed for simultaneous determination of 5 prohibited components
including cyclophosphamide, azacyclonol, aminopentamide, narcotine and metyrapene in different cosmetic matrices. The samples
of water emulsion and essential oil cosmetics were extracted with methanol, and the samples of cream cosmetics were dispersed
with saturated sodium chloride solution and extracted with anhydrous ethanol. Then both were purified with octadecyl silane boned
silica gel (C 18 ). The targets were separated on Waters BEH C 18 column using a mixed solution of 0. 1% (volume fraction) formic
acid solution and acetonitrile at different volume ratios as the mobile phase for gradient elution. Multiple reaction monitoring (MRM)
mode in mass spectrometry analysis was carried out under positive ion mode of electrospray ion source, and matrix matching
method was used for quantitative analysis. As shown by the results, linear relationships between the corresponding peak areas
− 1
and mass concentrations of 5 prohibited components were found in the range of 5-200 μg · L , with detection limits (3S/N)
in the range of 5-100 μg · kg −1 . Test for recovery at 3 concentration levels was conducted in different blank matrices, and the
recoveries of 5 prohibited components ranged from 90. 1% to 115%, with RSDs (n=6) of the determined values in the range of
2. 0%-14%.
Keywords: QuEChERS; ultra-high pressure liquid chromatography-triple quadrupole tandem mass spectrometry ; cosmetic;
prohibited component

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